recombinant nonstructural 3 protein, rns3, of hepatitis c virus along with recombinant gp96 induce il-12, tnfα and α5integrin expression in antigen presenting cells
نویسندگان
چکیده
conclusions we have highlighted the role of rns3 plus rnt-gp96 mediated by α5integrin in producing il-12 and tnfα. it can be suggested that rnt-gp96 could enhance immunity characteristic of rns3 protein via production of pro-inflammatory cytokines. results our results showed that rnt-gp96 alone significantly increases the expression level of il-12, tnfα and α5integrin in thp-1 macrophages and dcs, while il-12 and tnfα expression levels were unaffected by either rns3 or rrgd-ns3. interestingly, the co-addition of these recombinant proteins with rnt-gp96 increased il-12, tnfα and α5integrin expression. pearson correlation showed a direct association between α5integrin with il-12 and tnf-α expression. materials and methods recombinant viral proteins (rns3 and rrgd-ns3), n-terminal and c-terminal fragments of gp96 were produced and purified from e. coli in order to treat the cells; mouse spleen dendritic cells (dcs) and thp-1 macrophages. objectives the aim of this study was to examine additive effects of recombinant gp96 (rgp96) fragments accompanied by rns3 on expression levels of α5integrin and pro-inflammatory cytokines, il-12 and tnfα, in antigen presenting cells (apcs). background hepatitis c virus (hcv) infection is the main cause of chronic liver disease and to date there has been no vaccine development to prevent this infection. among non-structural hcv proteins, ns3 protein is an excellent goal for a therapeutic vaccine, due to its large size and less variation in conserved regions. the immunogenic properties of heat shock proteins (hsps) for instance gp96 have prompted investigations into their function as strong adjuvant to improve innate and adaptive immunity.
منابع مشابه
Recombinant Nonstructural 3 Protein, rNS3, of Hepatitis C Virus Along With Recombinant GP96 Induce IL-12, TNFα and α5integrin Expression in Antigen Presenting Cells
BACKGROUND Hepatitis C virus (HCV) infection is the main cause of chronic liver disease and to date there has been no vaccine development to prevent this infection. Among non-structural HCV proteins, NS3 protein is an excellent goal for a therapeutic vaccine, due to its large size and less variation in conserved regions. The immunogenic properties of heat shock proteins (HSPs) for instance GP96...
متن کاملConstruction and Eukaryotic Expression of Recombinant Large Hepatitis Delta Antigen
Background: Hepatitis delta virus (HDV) is a subviral human pathogen that exploits host RNA editing activity to produce two essential forms of the sole viral protein, hepatitis delta antigen (HDAg). Editing at the amber/W site of HDV antigenomic RNA leads to the production of the large form (L-HDAg), which is required for RNA packaging. Methods: In this study, PCR-based site-directed mutagen...
متن کاملConstructions of hepatitis C Virus prophylactic vaccine candidate using Berberis vulgaris stimulated and nonstructural protein 3 loaded dendritic cells
Introduction: Dendritic cells (DCs) have been recently employed as carriers for vaccines against several viral infections. The present study was designed to develop a prophylactic vaccine against hepatitis C virus (HCV) using DCs treated with Berberis vulgaris root extract (BRE), as a preclinical study. Methods: BRE was prepared and injected to female BALB/c mice for DCs expansion. The expanded...
متن کاملDownregulation of IL-12 Production in Healthy Non-Responder Neonates to Recombinant Hepatitis B Vaccine
A proportion of healthy neonates and adults fail to develop a protective antibody response to recombinant hepatitis B (HB) vaccine. Unresponsiveness to vaccination could be attributed to defect in a number of immunological regulatory mechanisms. In this study, IL-12 was quantitated in culture supernatant following in vitro stimulation of peripheral blood mononuclear cells isolated from a group ...
متن کاملInfluence of E1-Deleted Recombinant Adenoviruses on B7.1 and IL-2 Expression in C1498 Cells
Knowing that adenoviral vectors could initiate innate immunity, the ability of E1-deleted recombinant adenovirus (Ad-E1Δ) in induction of B7.1 and IL-2 molecules was studied. Methods: The expression of green fluorescent protein in C1498 cells following transfection of these cells with adenovirus green fluorescent protein vector confirmed the ability of adenovirus vectors in infecting the cells ...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
hepatitis monthlyجلد ۱۳، شماره ۶، صفحات ۰-۰
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023